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TransTaq-T DNA Polymerase (with 2.5 mM dNTPs)

Mixture of EasyTaq DNA Polymerase with a proofreading 3’-5’ exonuclease

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TransTaq-T DNA Polymerase (with 2.5 mM dNTPs)

Description

TransTaq-T DNA Polymerase is a mixture of EasyTaq DNA Polymerase with a proofreading 3’-5’ exonuclease.

The fidelity is equal to EasyPfu DNA Polymerase.

The yield is equal to that fromEasyTaq DNA Polymerase. It is more suitable for high fidelity TA cloning.


Highlights

  • TransTaq-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq DNA Polymerase
  • Extension rate is about 1-2 kb/min
  • Template-independent“A”can be generated at the 3’end of the PCR product
  • PCR products can be directly cloned intopEASY®-T vectors
  • Amplification of genomic DNA fragment up to 8 kb


Applications

  • Complex templates
  • TA cloning


Unit Definition

One unit of TransTaq-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

TransTaq-T DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.

Each batch of TransTaq-T DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers


Storage

  • At -20 ℃ for two years

Kit Contents

Component

TS/AP122/11

TS/AP122/12

TS/AP122/13

TransTaq-T DNA Polymerase

250 U×1

500 U×1

500 U×6

10×TransTaq-T Buffer

1.2 ml ×1

1.2 ml ×1

1.2 ml ×6

2.5 mM dNTPs

- / 400 µl ×1

- / 800 µl ×1

- / 800 µl ×6

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2


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