Choose your language: it-IT en-US
Facebook Twitter Instagram
« Come back | You are in: Skip Navigation Links Home » Life Science » Molecular Biology » Molecular Biology TS Series » PCR, RT-PCR, qPCR, qRT-PCR » PCR Enzyme »   TransStart FastPfu DNA Polymerase with 2 5 mM dNTPs

TransStart FastPfu DNA Polymerase (with 2.5 mM dNTPs)

Fast, high fidelity and high processivity hot start DNA polymerase

TransStart FastPfu DNA Polymerase (with 2.5 mM dNTPs)

Description

TransStart FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.


Highlights

• Extension rate is about 2-4 kb/min
• TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq®DNA Polymerase
• PCR products can be directly cloned into pEASY®-Blunt vectors
• Amplification of genomic DNA fragment up to 15 kb
• Amplification of plasmid DNA fragment up to 20 kb


Applications

• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates


Unit Definition

One unit of TransStart FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

TransStart FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.

Each batch of TransStart FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol


Storage

At -20 ℃ for two years


Kit Contents

Component

TS/AP221/11

TS/AP221/12

TS/AP221/13

TransStart® FastPfu DNA Polymerase

250 U×1

500 U×1

500 U×6

5×TransStart FastPfu Buffer

1.2 ml×1

1.2 ml ×2

1.2 ml ×12

2.5 mM dNTPs

- / 500 µl×1

- / 1 ml ×1

- / 1 ml ×6

50 mM MgSO4

200 µl×1

400 µl ×1

1 ml ×1

PCR Stimulant

200 µl×1

400 µl×1

1 ml ×1

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2


You might also be interested in these items
all rights reserved © 2017 CHEIMIKA SAS DI HEIM JURGENYour purchases are guaranteed by the major lenders
Powered by NapoliWeb S.r.l. - Web Agency Napoli Web Agency Napoli