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EasyTaq DNA Polymerase for PAGE

Purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus

EasyTaq DNA Polymerase for PAGE (dNTPs-free)

Description

EasyTaq DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus.

The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa.

EasyTaq DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity.

This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.


Highlights

  • Extension rate is about 1-2 kb/min
  • Unique buffer system compatible with PAGE
  • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned intopEASY-T vectors
  • Amplification of genomic DNA fragment up to 3 kb


Applications

Short fragment PCR


Unit Definition

One unit of EasyTaq DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

EasyTaq DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.

Each batch of EasyTaq DNA Polymerase for PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers


Storage

  • At -20°C for two years


Kit Contents

Component

TS/AP112/01 

TS/AP112/02

EasyTaq® DNA Polymerase for PAGE

2500 U

2500 U×4

10×EasyTaq® Buffer for PAGE

1.2 ml ×5

1.2 ml×20

6×DNA Loading Buffer

1 ml

1ml ×4


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