TransScript II One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover

Description

Unique genomic DNA remover is combined with TransScript II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.

After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

Highlights

Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
cDNA up to 15 kb

Applications

cDNA library construction, 3’ and 5’ RACE
Multiple copy and low copy gene detection
GC-rich or complex secondary structure RNA template

Storage

At -20℃ for two year

Kit Contents

Component	TS/AH311/02 (50 rxns)	TS/AH311/03 (100 rxns)
TransScript II RT/RI Enzyme Mix	50 μl	100 μl
gDNA Remover	50 μl	100 μl
2×TS II Reaction Mix	500 μl	1 ml
Random Primer (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml

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