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2×TransTaq High Fidelity(HiFi)PCR SuperMix II (-dye )

Ready-to-use mixture of TransTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer

2×TransTaq High Fidelity(HiFi)PCR SuperMix II (-dye)

Description

TransTaq High Fidelity (HiFi) PCR SuperMix II is a ready-to-use mixture of TransTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer.

TransTaq High Fidelity (HiFi) PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

• TransTaq High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase
• Extension rate is about 1-2 kb/min
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY-T vectors
• Amplification of genomic DNA fragment up to 15 kb

Applications

• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR

Storage

At -20℃ for two years

Kit Contents

Component

TS/AS131/21

TS/AS131/22

2×TransTaq HiFi PCR SuperMix II

1 ml

5×1 ml

ddH2O

1 ml

5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×TransTaq HiFi PCR SuperMix II

25 μl

ddH2O

Variable

-

Total volume

50 μl

-


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