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TransTaq DNA Polymerase (HiFi)(with 2.5 mM dNTPs)

Provides higher specificity and higher amplification efficiency

TransTaq DNA Polymerase High Fidelity (HiFi) - (with 2.5 mM dNTPs)

Description

TransTaq DNA Polymerase High Fidelity (TransTaqHiFi DNA Polymerase) contains TransTaq-T DNA Polymerase and a proofreading 3’-5’ exonuclease.

TransTaq HiFi DNA Polymerase provides higher specificity and higher amplification efficiency thanTransTaq-T DNA Polymerase.

Two different buffers are provided in the kit.TransTaq HiFi Buffer I is optimized for the amplification of genomic DNA andTransTaq HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.


Highlights

  • TransTaq HiFi DNA Polymerase offers 18-fold fidelity as compared toEasyTaq DNA Polymerase
  • Extension rate is about 1-2 kb/min
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned intopEASY-T vectors
  • Amplification of genomic DNA fragment up to 15 kb


Applications

  • Complex templates
  • GC/AT rich templates
  • Long PCR
  • High yield PCR

Unit Definition

One unit of TransTaq HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

TransTaq HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.

Each batch ofTransTaq HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.


Storage

At -20 ℃ for two years

Kit Contents

Component

TS/AP131/11

TS/AP131/12

TS/AP131/13

TransTaq® HiFi DNA Polymerase

250 U×1

500 U×1

500 U×6

10×TransTaq® HiFi Buffer I

1.2 ml×1

1.2 ml×1

1.2 ml×6

10×TransTaq® HiFi Buffer II

1.2 ml×1

1.2 ml×1

1.2 ml×6

2.5 mM dNTP

-/400 μl×1

-/800 μl×1

-/800 μl×6

10×GC Enhancer

200 μl×1

400 μl×1

1 ml×1

6×DNA Loading Buffer

500 μl×1

1 ml×1

1 ml×2


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