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TransScript II First-Strand cDNA Synthesis SuperMix

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

TransScript II First-Strand cDNA Synthesis SuperMix

Description

TransScript II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA.

The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix.

  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
  • Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA
  • cDNA up to 15 kb

Applications

  • cDNA library construction, 3’ and 5’ RACE
  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNA template

Storage

At -20℃ for two year

Kit Contents

Component

TS/AH301/02

TS/AH301/03

TransScript II RT/RI Enzyme Mix

50 µl

100 µl

2×TS II Reaction Mix

500 µl

1 ml

Random Primer (N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo(dT)20 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml


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