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TransScript Reverse Transcriptase

Recombinant M-MLV reverse transcriptase with deficient RNase H activity.

TransScript Reverse Transcriptase

Description

TransScript Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
  • Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA
  • cDNA up to 12 kb

Applications

  • First-strand cDNA synthesis
  • Multiple copy and low copy gene detection

Unit Definition

One unit of TransScript RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

5×TS RT Buffer

250 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4

Storage

At -20℃ for two year

Kit Contents

Component

TS/AT101/02

TS/AT101/03

TransScript RT

10000 U

5×10000 U

5×TS RT Buffer

200 μl

5×200 μl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 μl

250 μl


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