TransScript-Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

improved version of M-MLV reverse transcriptase with higher thermostability.

Description

TransScript-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.

Highlights

Broad range reaction temperature (42°C -65°C )
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
cDNA up to 20 kb

Applications

Multiple copy and low copy gene detection
GC-rich or complex secondary structure RNA template
cDNA library construction, primer extension, 3’ and 5’ RACE

Storage

At-20°C for two year

Kit Contents

Component	TS/AU311/02	TS/AU311/03
TransScript® -Uni RT/RI Enzyme Mix	50 μl	100 μl
gDNA Remover	50 μl	100 μl
2×TS-Uni Reaction Mix	500 μl	1 ml
Random Primer(N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml

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