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BRAIN HEART AGAR

Recommended for the development of fastidious microorganisms

BRAIN HEART INFUSION AGAR (BHI AGAR) - 500 g

Recommended for the development of fastidious microorganisms 

Applications

  • Maintenance of strains
    Media for general use
    OTHER APPLICATIONS

Packaging

Suspend 52 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize at 121ºC for 15 minutes. To prepare a selective medium for fungi, the sterilized and melted medium should be cooled to 45 – 50°C before adding the appropriate antibiotics. Occasionally a small amount of sediment may appear which should be resuspended with a gentle swirl before dispensing.

Formula

  • Peptone Mixture 10
  • Beef Heart Infusion 10
  • Calf Brain Infusion 7.5
  • Sodium Chloride 5
  • Disodium Phosphate 2.5
  • Dextrose 2
  • Bacteriological Agar 15

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