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BOLTON BROTH

For the selective preenrichment of Campylobacter in foods samples

BOLTON SELECTIVE ENRICHMENT BROTH BASE ISO 10272 - 500 g

For the selective preenrichment of Campylobacter in foods samples

Applications

  • Selective isolation
    CAMPYLOBACTER

Packaging

Suspend 13.8 grams of the medium in 500 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of the Bolton Selective Supplement (Cat. 6070) reconstituted in 5 ml of sterile distilled water and 25 ml of sterile laked blood. Homogenize gently and dispense into Petri dishes.

Formula

  • Meat Peptone 10
  • Lactalbumin Hydrolysate 5
  • Yeast Extract 5
  • Sodium Chloride 5
  • Alpha-ketoglutaric Acid 1
  • Sodium Piruvate 0.5
  • Sodium Metabisulfate 0.5
  • Sodium Carbonate 0.6
  • Hemin 0.01

Recommended supplement (to be purchased separately)

  • BOLTON SELECTIVE SUPPLEMENT - Ready to use 1 X 10 Vials. Each vial for 500 ml. Of medium

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