TransScript II Reverse Transcriptase
Description
TransScript II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability.
The enzyme is active at up to 55℃.
It provides higher specificity, higher yield and more full-length cDNA products.
Applications
Unit Definition
One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.
10×TS II RT Buffer
500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4
Storage
At -20℃ for two year
Kit Contents
Component
TS/AH101/02
TransScript® II RT
10.000 U
100 μl
Anchored Oligo(dT)20 Primer (0.5 µg/µl)
50 μl