TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover

Scheda tecnica disponibile solo in lingua inglese.

Unique genomic DNA remover is combined with TransScript First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.

After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

Highlights

Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination
The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
cDNA up to 12 kb

Applications

Multiple copy and low copy gene detection

Storage

At -20℃ for two year

Kit Contents

Component	TS/AT311/02 (50 rxns)	TS/AT311/03 (100 rxns)
TransScript® RT/RI Enzyme Mix	50 μl	100 μl
gDNA Remover	50 μl	100 μl
2×TS Reaction Mix	500 μl	1 ml
Random Primer(N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml

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