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TransScript First-Strand cDNA Synthesis SuperMix

Provides all the necessary components for cDNA synthesis from total RNA or mRNA

 Scheda tecnica disponibile solo in lingua inglese.

TransScript First-Strand cDNA Synthesis SuperMix

Description

TransScript First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript RT/RI Enzyme Mix and 2xTS Reaction Mix.

  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR
  • Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
  • cDNA up to 12 kb

Applications

Multiple copy and low copy gene detection

Storage

At -20℃ for two year

Kit Contents

Component

PT/AT301/02

PT/AT301/03

TransScript® RT/RI Enzyme Mix

50 µl

100 µl

2×TS Reaction Mix

500 µl

1 ml

Random Primer (N9) (0.1 µg/µl)

50 µl

100 µl

Anchored Oligo (dT)18 Primer (0.5 µg/µl)

50 µl

100 µl

RNase-free Water

500 µl

1 ml

 


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