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EasyPfu DNA Polymerase (with 2.5 mM dNTPs)

Engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity

 Scheda tecnica disponibile solo in lingua inglese.

EasyPfu DNA Polymerase (with 2.5 mM dNTPs)


EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.


• EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.


• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis

Unit Definition

One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

Quality Control

EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE.

Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers


At -20°C for two years

Kit Contents





EasyPfu DNA Polymerase

250 U×1

500 U×1

500 U×6

10×EasyPfu Buffer

1.2 ml ×1

1.2 ml ×1

1.2 ml ×6

2.5 mM dNTPs

- / 500 µl ×1

- / 1 ml ×1

- / 1 ml ×6

6×DNA Loading Buffer

500 μl×1

1 ml ×1

1 ml ×2

50 mM MgSO4

200 μl×1

400 μl×1

1 ml ×1

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